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Figure 3 | BMC Genomics

Figure 3

From: Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis

Figure 3

2D-PAGE analysis of prefractionated S. pombe cell lysate (A) Workflow for fractionation using Vivapure Q spin columns by applying increasing NaCl concentrations for elution. See Methods for details. (B) Reproducibility of the prefractionation. Two independently prepared whole cell lysates were prefractionated on Vivapure Q Mini columns and eluted into three fractions with the indicated concentrations of NaCl. The protein contents of the eluates are plotted. (C) 2D-PAGE of the three fractions compared to whole cell lysate.

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BMC Genomics

ISSN: 1471-2164

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