2D-PAGE analysis of prefractionated S. pombe cell lysate (A) Workflow for fractionation using Vivapure Q spin columns by applying increasing NaCl concentrations for elution. See Methods for details. (B) Reproducibility of the prefractionation. Two independently prepared whole cell lysates were prefractionated on Vivapure Q Mini columns and eluted into three fractions with the indicated concentrations of NaCl. The protein contents of the eluates are plotted. (C) 2D-PAGE of the three fractions compared to whole cell lysate.