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Table 1 Putative alternate splice variants of MYB and RT-PCR results testing their existence.

From: Genome annotation of a 1.5 Mb region of human chromosome 6q23 encompassing a quantitative trait locus for fetal hemoglobin expression in adults

  Evidence    
Putative Alternate exon Literature Existing sequence EST Evidence Ensembl transcript Gene Prediction Conserved region with mouse RT-PCR primer sequences: Positive result by RT-PCR (PCR conditions) Sequence Accession Number
1A        F: GCCCGAAGACCCCGGCACAG
R:TGCAACTTCCCAGCGCCGCG
  
1B       F: GCCCGAAGACCCCGGCACAG
R:CCAACAGTCAGAGGATTTTCAAAG
  
1C       F: GCCCGAAGACCCCGGCACAG
R:CAATGCATCCAGCAATTACG
  
1D       F: GCCCGAAGACCCCGGCACAG
R:GGCCACTTGTTAGTCAGAGTCTT
  
1E        F: GCCCGAAGACCCCGGCACAG
R:TACACATTTGGCCACCTTCC
  
2' [22] X52125      F: GCCCGAAGACCCCGGCACAG
R:TGCAGAAATAAGAATGGGTAGACG
  
5A        F: GCCCTGCTGTGCCACATTCAAA
R:CGGAGCCTGAGCAAAACCCATC
  
8' [22] X52125     F: AAATATAGTCAATGTCCCTCAGCC
R:ATGTGTGGTTCTGTGTCTGCTGT

2.5 mM/60C
AJ606320
8-extended [23] M13666     F: AAATATAGTCAATGTCCCTCAGCC
R:ACAAAATAATAGAAAAAAACCAAAATG
  
8A [22] U22376, X52125      F: AAATATAGTCAATGTCCCTCAGCC
R:GGGCATCACTTCTCTTTTATTGTCT

1.5 mM, 60C
AJ606317
8A-extended       F: AAATATAGTCAATGTCCCTCAGCC
R:GCACATGCTAGTTCGACCAC
  
9Ai   U22376      F: ACAGAACCACACATGCAGCTACC
R:TGAAACAGCACTGAGAGAGAGATG

2.5 mM, 65C
AJ606318
9Aii [21,22] X17469, X52126, U22376   F: AAATATAGTCAATGTCCCTCAGCC
R:ACAGGGGAACGCTTGGGAGT

1.5 mM, 62C
AJ606319
9B       F: ACAGAACCACACATGCAGCTACC
R:CACAATTTGGTTCCCTCCTC
  
9C       F: ACAGAACCACACATGCAGCTACC
R:TATTCCTTGGCAAAAACTGC
  
9D       F: ACAGAACCACACATGCAGCTACC
R:GGTCATTGCTGAAAAACTTGC
  
10A   U22376      F: ACAGAACCACACATGCAGCTACC
R:CATCCCTTGGCTTCTAATCATATAA

2.5 mM, 55C
AJ606322
11'        F: ACAGAACCACACATGCAGCTACC
R:CCTTTTGATAGCTGGGGTTACAGT
  
13A   U22376      F: AAACTTCTTCTGCTCACACCACTG
R:CTCACGCCTGTAATCCTAGCAC

1.5 mM, 60C
AJ606323
14'        F: AAACTTCTTCTGCTCACACCACTG
R:AAATACCAATGTTGGAATAGTAAT
  
14A       F: AAACTTCTTCTGCTCACACCACTG
R:TGGCTCAGGATTAATTTGGAA

2.5 mM, 50C
AJ606324
14B       F: AAACTTCTTCTGCTCACACCACTG
R:TGCAATATGTTCAATAATACCATGTG
  
14C       F: AAACTTCTTCTGCTCACACCACTG
R:TCTCGCTTATCCTGTAATGTGC
  
  1. The putative alternate splice variant is indicated in the 1st column, and the subsequent columns indicate lines of evidence that suggested the existence of this splice variant. The literature references and accession numbers for previously reported splice variants are provided. Ensembl transcripts were observed on the v.9.30a.1 (2/12/2002) Ensembl release. Gene predictions were suggested by Genscan, Fgenesh++ or Twinscan, and conserved regions were classified as intronic sequence of >70% homology over 100 bp with the syntenic mouse region. The RT-PCR primer sequences used to test the existence of each alternate exon are listed, followed by a column indicating whether this splice variant was detectable by RT-PCR, and the associated PCR conditions. The final column provides the accession number of our sequences for the confirmed alternate splice variant.