Genome annotation of a 1.5 Mb region of human chromosome 6q23 encompassing a quantitative trait locus for fetal hemoglobin expression in adults

BMC Genomics

Table 2 Experimentally verified genes and transcripts identified through EST based evidence.

Gene	Transcript	Acc. No.	Length	Expression verified in:	Number of exons	Longest ORF
1	1	AJ606314	831 bp	Testes	6	138 bp
	2	AJ606325	717 bp	Testes	5	81 bp
	3	AJ606326	636 bp	Testes	4	150 bp
	4	AJ606327	522 bp	Testes	5	150 bp
2	1	AJ606328	2017 bp	Testes, Bone Marrow	5	294 bp
	2	AJ606329	1553 bp	Testes, Bone Marrow	3	294 bp
	3	AJ606330	1595 bp	Testes, Bone Marrow	5	294 bp
3	1	AJ606331	2501 bp	Testes, Placenta	10	258 bp
4	1	AJ606332	2106 bp	Testes	3	213 bp
	2	AJ606315	2390 bp	Testes	5	150 bp
	3	AJ606316	2718 bp	Testes, Placenta	3	210 bp

The genes have been named 1–4 for the purposes of this paper, with each gene being subdivided into separate transcripts (e.g. splice variants). The "Expression verified in" column lists which tissues we have verified the expression of the transcripts by RT-PCR (from a cDNA panel including Fetal Liver, Erythroid, Lymphoblastoid cell line, Spleen, Stomach, Thymus, Small Intestine, Colon, Bone Marrow, Placenta and Testes). The final column lists the longest Open Reading Frame identified in each cDNA, defined as the longest uninterrupted stretch between a methionine and a stop codon.