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Table 2 The effect of oligonucleotide length and concentration on average signal intensity ratios.

From: Improving signal intensities for genes with low-expression on oligonucleotide microarrays

Experimental Condition   Average Intensity Ratio
   Low-expressing genes* High-expressing genes*
Oligonucleotide Length @ 50 μM 70 22.1 ± 13.6 7.4 ± 5.4
  50 6.1 ± 3.9 2.7 ± 1.2
  40 2.4 ± 1.3 2.0 ± 0.8
Oligonucleotide Concentration (μM) for length 70 50 3.1 ± 1.8 1.2 ± 0.2
  40 1.9 ± 1.1 0.9 ± 0.2
  30 1.5 ± 0.6 1.1 ± 0.1
  1. * Low- and High-expressing genes are categorized based on relative expression in RKO cell line as shown in Table 1 and definitions are in results and discussion.