Figure 1From: SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones Library construction process Schematic representation of the library construction process. Fist strand cDNA is synthesized with the SMART system (1), second strand synthesis is primed by a SMART oligo-specific primer (2), double-stranded cDNA is size-fractionated via agarose gel electrophoresis (3), and size fractions are amplified and cloned separately (4).Back to article page