Figure 4

Detection of biologically meaningful gene regulation. (3A) Core reference spots showing significant up-regulation in the brain decreases with phylogenetic distance. All 804 spots were examined in each species. The Y-axis depicts the percentage of the core reference set spots that were identified as significantly up-regulated in the brain of each species. Venn diagrams depict the relationship of identified spots from the core reference set as re-identified in each species. The number of A. burtoni reference set spots that are also significantly up-regulated in the brains of (3B) other cichlids and (3C) distantly related fish are shown in the appropriately represented area. 108 and 302 spots are not re-identified in the cichlids or distantly related fish respectively (not shown).