Figure 4

A Cartoon representation of the YjeF-N type Rossmann fold and its conserved features. The cartoon representation of the YjeF-N-type Rossmann fold domain was constructed using the crystal structure of the yeast YjeF-N domain containing protein (PDB: 1JZT). The N terminal helices are named N1 and N2, and the core helices and strands are named H1 to H7 and S1 to S8 respectively. The conserved residues of this fold corresponding to D16, E33, N69, N70, R79, H80, D138, D173 and T176 in this fold are shown in ball and stick representation. The salt bridges (E33 and R79 and H80) and hydrogen bonds (D138 and T176) between these conserved residues that are critical for the stabilization of the fold are shown as magenta dotted lines. The region between the strand 1 and helix 1 of the α/β core that corresponds to the glycine-rich nucleotide binding loop in the classic Rossmann fold (residues 66 and 72) is shown in red. Note the curvature of the central sheet and the packing of helix 1 of the α/β core and the second N-terminal additional helix.