Figure 3
From: Expression profile of genes regulated by activity of the Na-H exchanger NHE1
Genes differentially regulated in LAPE cells grouped as functioning in the G2/M transition of cell cycle progression and DNA damage checkpoint. A. Schematic diagram of G2/M regulation. Red indicates genes with increased expression in LAPE cells compared with LAPN cells, and blue indicates genes with decreased expression. B. Immunoblot of GADD45 confirmed decreased protein expression in LAPE cells compared with LAPN cells. C. Immunblotting for FEN1 and cyclin B1 at the indicated times after release from a double thymidine block. C. Relative TaqMan expression of Wee1 kinase confirmed GeneChip data of increased Wee1 expression in LAPE cells compared with LAPN cells. Data in A represent the means of fold-increase or – decrease in LAPE cells (p < 0.05, n = 5). Data in B and C are representative of 2 to 3 separate cell preparations. Data in D represent the mean ± s.e.m. of 3 separate cell preparations.