Table 1 Primers for nested PCR analyses
Sequence from exon | Outer PCR primer pairs | Inner PCR primer pairs | |
|---|---|---|---|
8 | fwd | 5'-AACATCCTCCTCGCCAAGGGT | 5'-ATATGAACTGGCAGTAGGCAC |
rev | 5'-TGCTCTCCTGACTGT GCCTGAGTAGACTCA | 5'-TTACCCTTGGGGGCCAACCGA | |
13 | fwd | 5'-AACATCCTCCTCGCCAAGGGT | 5'-AGCCTGTGCCTATTCAACTGA |
rev | 5'-TGCTCTCCTGACTGT GCCTGAGTAGACTCA | 5'-GCCTCCCGGCAGAAGGAATAC | |
24 | fwd | 5'-CGCAAGTGAGCCAGCCACTGC | 5'-CAGCCAGCTCAGGCCATCCCT |
rev | 5'-CTGGACCGCAGGAATCT GGAATCACATGCTATGGG | 5'-CCAGGCCTGTGAGAAGGCTGA | |