Phylogenetic relationships between fish and human CTLDs. A phylogenetic tree built on a ClustalW alignment of a 95% non-redundant collection of predicted Fugu CTLDs and known human and fish CTLDs. Link domains and group VI CTLDs were excluded from the alignment. The tree was built by the neighbor-joining method with 100 bootstrap trials using the ClustalW program. PhyloDraw was used to draw the radial cladogram shown. Branches containing CTLDs from CTLDcps belonging to the same group are shaded; group numbers are marked. Lower case prefixes in the identifiers indicate taxonomic origin: h – Homo sapiens, f – Fugu rubripes, zbrfs – Danio rerio (zebrafish), g – Gillichthys mirabilis, gldhs – Carassius auratus (goldfish), carp – Cyprinus carpio (common carp), rsmlt – Osmerus mordax (rainbow smelt), slmn – Salmo salar (Atlantic salmon), wfldr – Pseudopleuronectes americanus (winter flounder), ahrng – Clupea harengus (Atlantic herring), servn – Hemitripterus americanus (sea raven), jpeel – Anguilla japonica (Japanese eel), medak – Oryzias latipes (Japanese medaka), c – Paralabidochromis chilotes (cichlid fish).