Figure 2

Long motifs specific of putrescine carbamoyltransferases. The amino acid sequence of the open reading frame EF0732 of E. faecalis annotated as ArgF-2 [14] but now reannotated as putrescine carbamoyltransferase is shown. The NH₂-terminal 29 residues sequence [13] which helped to this reannotation is indicated by a red bar above the respective residues. The amino acids which are specifically conserved in putrescine carbamoyltransferases and not in ornithine carbamoyltransferases are printed in bold. Inside the long motifs (> 15 residues) found along the whole sequence and numbered as "Motif 1" to Motif 5"are indicated the few substitutions present in the other sequences (lines above the E. faecalis sequence) including those of M. mycoides which is more distant (line in italic below the E. faecalis sequence). The residues forming the respective binding sites of carbamoylphosphate and of either putrescine or ornithine are also in bold and framed.