Figure 1
From: Immobilized probe and glass surface chemistry as variables in microarray fabrication
Evaluation of measured output ratio of spiked Arabidopsis in vitro transcript at known input ratios. A. Total thymus RNA extracted from the DR+/+ and DRlyp/lyp [6] BioBreeding rats spiked with known input ratios of Arabidopsis gene in vitro transcript and hybridized to 18,000 probe rat cDNA arrays possessing serially diluted fluorescein-labeled Arabidopsis probes. B. Evaluation of data compression as a function of support-bound probe. On the x-axis is plotted the average pixel fluorescein intensity per spot plotted against the Arabidopsis transcript measured output log ratio/actual input log ratio. As spot intensities fall below 5000 RFU/pixel, ratio measurements become increasingly compressed.