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Table 9 Presumptive human chromosome 17 loci that were mapped in Ambystoma

From: From biomedicine to natural history research: EST resources for ambystomatid salamanders

Marker ID Primersa Diagnosisb LGc Symbold RefSeq IDe E-valuef
Pl_6_E/F_6 F-GAAAACCTGCTCAGCATTAGTGT ASA ul PFN1 NP_005013 E-34
  R-TCTATTACCATAGCATTAATTGGCAG      
Pl_5_G/H_5 F-CTATTTCATCTGAGTACCGTTGAATG PE (A) 23 CGI-125 NP_057144 E-56
  R-TAATGTAGAACTAAATGGCATCCTTC      
  E-CCATGGTGCAGGAAGAGAGCCTATAT      
Pl_0.4_A/B_1 F-GTCTCATTATCCGCAAACCTGT SP 1 RPL19 NP_000972 E-67
  R-ATTCTCATCCTCCTCATCCACGAC      
Pl_4_B_7/8 F-CCTAGAACATTACCAAAACAGACTCA RD (Dpn II) 1 KRT10 NP_061889 E-17
  R-AATGAAGAAGTATTGCATGTGAGAAC      
Pl_4_B_9/10 F-GAACTTGTTGGCAGGTTTCTCTT RD (AciI) 1 KRT17 NP_000413 E-146
  R-CTAGTGATAGGTTGGACATACCAGAG      
Pl_10_C/D_4 F-CTCCACTATTTAAAGGACATGCTACA PE (A) 1 SUI1 NP_005792 E-48
  R-TTAATATAGCACAACATTGCCTCATT      
  E-TGCTACATTAATGTAATAAACGGCATCATC      
Pl_6_E/F_11 F-AAGAGAAGTTCCTAGATGAGTTGGAG PE (A) 1 HSPC009 NP_054738 E-26
  R-TGAAGAGAGAACTCAAAGTGTCTGAT      
  E-TCATGTTTTGCTCTGCTGTGCAGT      
Pl_9_A/B_10 F-TGATAGTTTCTGGATTAAGACGAGTG PE (T) 1 FLJ13855 NP_075567 E-15
  R-CTTAGAGCCATTGTTACAAGATGTTC      
  E-GTGATCTAGTGGGATCAAACCCTAAAGACC      
Pl_10_C/D_9 F-AAAGTGCCAAGAAGGAGATTAACTT PE (T) 9 NME1 NP_000260 E-71
  R-GAGCTCAGAAAACAAGGCAGTAAC      
  E-AAATGGATCTACGAGTAGACCTTGACCC      
Pl_9_C/D_9 F-GAGTCTCCTTTAGGATTGACGTATCT PE (T) 23 FLJ20345 NP_060247 E-17
  R-GCTATGTGAGCAGAGATAAAAGTCAG      
  E-GTTACAGCATCAGTGGGATGTGGTATGT      
Pl_8_C/D_9 F-AGGATACCAACCTCTGTGCTATACAT PE (C) 15 H3F3B NP_005315 E-66
  R-TAAATGTATTTACAAACCGAAAGCAA      
  E-CGTGGCGAGCGTGCCTAGT      
Pl_9_C/D_4 F-GTGGTTATTTGTAACATTTCGTTGAC PE (A) 8 SFRS2 NP_003007 E-40
  R-AATTACATTTGGGCTTCTCAATTTAC      
  E-TTTTTAAACGCGTAAAAATGTTAACAGA      
Pl_6_C/D_5 F-CCGTAAATGTTTCTAAATGACAGTTG PE (G) 2 ACTG1 NP_001605 0
  R-GGAAAGAAAGTACAATCAAGTCCTTC      
  E-GATTGAAAACTGGAACCGAAAGAAGATAAA      
  1. aSequences are 5' amplification primers, 3' amplification primers, or primer extension probes, and are preceded by F-, R-, and E- respectively. bGenotyping methods are abbreviated: allele specific amplification (ASA), size polymorphism (SP), restriction digestion (RD), primer extension (PE). Diagnostic restriction enzymes and diagnostic extension bases are provided in parentheses. cAmbystoma linkage group ID. "ul" designates markers that are unlinked. dOfficial gene symbols as defined by the Human Genome Organization Gene Nomenclature Committee http://www.gene.ucl.ac.uk/nomenclature/. eBest BLASTX hit (highest e-value) from the human RefSeq database using the contig from which each marker was designed as a query sequence. fHighest E-value statistic obtained by searching contigs, from which EST markers were designed, against the human RefSeq database.