Figure 5

Absolute and normalized expression of gp100 based on qRT-PCR. Four melanoma cell lines expressing gp100 (MEL-553B, MEL 1317, MEL 526 and MEL-1102) and 2 not expressing gp100 (MEL-836 and MEL-1195) protein by FACS analysis [35] (maroon horizontal bar) were tested for gp100 expression by qRT-PCR as previously described [40]. In addition four fine need aspirates of melanoma metastases all expressing GP100 by immunohistochemistry (orange horizontal bars) and two renal cell, two esophageal and one gastric cancer specimens all not expressing gp100 (green horizontal bar) were tested. Similarly the gp100 not expressing HeLa cell line was tested. Absolute gp100 copy number is shown in (a); normalized expression of gp100 is shown using the average of all endogenous reference gene results (b). The protein expression for the melanoma cell lines is presented as mean equivalent of fluorescence (MEF) [35] as well as immunohistochemical evaluation while the expression by FNA is shown in (c) based on immunohistochemical (HIC) evaluation as previously described [46]. Absolute copy numbers of gp100 estimated by qRT-PCR are also shown.