A genome-wide screen identifies a single β-defensin gene cluster in the chicken: implications for the origin and evolution of mammalian defensins

Table 2 Primer sequences used for RT-PCR analysis of novel chicken β-defensins

Gene	Primer Sequence		Product Size (bp)		Cycles Used
	Sense	Antisense	cDNA	Genomic
Gal4	CATCTCAGTGTCGTTTCTCTGC	ACAATGGTTCCCCAAATCCAAC	321	899	36
Gal5	CTGCCAGCAAGAAAGGAACCTG	TGAACGTGAAGGGACATCAGAG	300	1100	36
Gal6	AGGATTTCACATCCCAGCCGTG	CAGGAGAAGCCAGTGAGTCATC	249	1203	36
Gal7	CTGCTGTCTGTCCTCTTTGTGG	CATTTGGTAGATGCAGGAAGGA	230	665	35
Gal8	ACAGTGTGAGCAGGCAGGAGGGA	CTCTTCTGTTCAGCCTTTGGTG	261	967	35
Gal9	GCAAAGGCTATTCCACAGCAG	AGCATTTCAGCTTCCCACCAC	211	1802	33
Gal10	TGGGGCACGCAGTCCACAAC	ATCAGCTCCTCAAGGCAGTG	298	2285	33
Gal11	ACTGCATCCGTTCCAAAGTCTG	TCGGGCAGCTTCTCTACAAC	301	1299	33
Gal12	CCCAGCAGGACCAAAGCAATG	GTGAATCCACAGCCAATGAGAG	335	731	36
Gal13	CATCGTTGTCATTCTCCTCCTC	ACTTGCAGCGTGTGGGAGTTG	175	4514	50
GAPDH	GCACGCCATCACTATCTTCC	CATCCACCGTCTTCTGTGTG	356	876	30

ISSN: 1471-2164