Figure 3
From: Combined analysis of expression data and transcription factor binding sites in the yeast genome
a 1-α 2 binding in vitro and in vivo to sites in the ORF regions of the genome. (A) An EMSA of purified a 1 and α 2 proteins binding to a strong a 1-α 2 binding site, HO(10) (lanes 1–8) and sites from the YKL162C (lanes 9–13), CDC25 (lanes 14–18), a weak a 1-α 2 binding site from the HO promoter, HO(8) (lanes 19–23), PRM8 (lanes 24–28), PRM9 (lanes 29–33) and URB1 (lanes 34–38). The concentration of the a 1 protein was held constant at 1.4 × 10-6 M (lanes 2 and 4–38) and mixed with 5-fold dilutions of the α 2 protein starting at 8.2 × 10-8 M (lanes 3, 4, 9, 14, 19, 24, 29 and 34)). The EMSAs shown are phosphorimages of the gels. Lane 1 contains HO(10) probe alone, and lanes 2 and 3 contain 1.4 × 10-6 M a 1 and 8.2 × 10-8 M α 2 respectively. The fold repression by each site in the context of a heterologous promoter is shown below each gene/promoter. (B) ChIP assays for the genes YKL162C, CDC25, PRM8, PRM9 and URB1 are shown. ChIP assays were performed as described in Figure 1