Figure 4
From: Combined analysis of expression data and transcription factor binding sites in the yeast genome
a 1-α 2 binding in vitro and in vivo to putative binding sites in promoters associated with genes which are not expressed in a haploid-specific manner. (A) An EMSA of purified a 1 and α 2 proteins binding to a strong a 1-α 2 binding site, HO(6) (lanes 1–7), COX13 (lanes 8–11), REX2 (lanes 12–15), LSM1 (lanes 16–19) and FMP14 (lanes 20–23). The concentration of the a 1 protein was held constant at 1.4 × 10-6 M (lanes 2 and 4–23) and mixed with 5-fold dilutions of the α 2 protein starting at 8.2 × 10-8 M (lanes 3, 4, 8, 12, 16 and 20). The EMSAs shown are phosphorimages of the gels. Lane 1 contains HO(6) probe alone, and lanes 2 and 3 contain 1.4 × 10-6 M a 1 and 8.2 × 10-8 M α 2 respectively. The fold repression by each site in the context of a heterologous promoter is shown. (B) ChIP assays for COX13, REX2, LSM1 and FMP14 were performed as described in Figure 1.