Figure 2

EF1α promoter truncations and endogenous enhancer trap screening. A diagram of the S1 EF1α promoter [32, 41]. Restriction enzyme sites are shown on top as single letters. S is Sph I, N is Nhe I, B is Bst 1107I and R is Eco RI. G/C, G and C rich box. Sp1, Sp1-like site. TATA, TATA box. Numbering below is relative to the first T of the TATA box. The table below the diagram shows the results of the pilot and scale-up (*) screens. Transgenesis and expression rates are shown, non-expressing transposon insertions were not scored. Transgenesis and expression rate from scale-up screen (^#) is an underestimate since many founders were screened by incross and crosses from doubly transgenic founders were scored as a single transmission event (see text).