Skip to main content

Table 1 Summary of database contents. A summary of the data sets included in the initial release of PEDRo. The database provides more detailed descriptions of sample generation, sample processing, mass spectrometry and in silico analyses, populating the model described in [2].

From: PEDRo: A database for storing, searching and disseminating experimental proteomics data

1

Organism

Saccharomyces cerevisiae

Sample Generation

Whole cell extracts (bead beating)

 

Experimenter

Al Brown et al.

Sample Processing

2D gel

 

Description

GCN4-dependent proteins that respond to amino acid starvation

Mass Spectrometry

MALDI-ToF

   

In Silico Analysis

Peptide mass fingerprint searches (MASCOT, MS-Fit)

2

Organism

Candida albicans

Sample Generation

Whole cell extracts (bead beating)

 

Experimenter

Al Brown et al.

Sample Processing

2D gel

 

Description

GCN4-dependent proteins that respond to amino acid starvation

Mass Spectrometry

MALDI-ToF

   

In Silico Analysis

Peptide mass fingerprint searches (MASCOT, MS-Fit)

3

Organism

Candida glabrata

Sample Generation

Whole cell extracts (bead beating)

 

Experimenter

Ken Haynes et al.

Sample Processing

2D gel

 

Description

Proteins that respond to the inactivation of ACE2

Mass Spectrometry

MALDI-ToF

   

In Silico Analysis

Peptide mass fingerprint searches (MASCOT, MS-Fit)

4

Organism

Streptomyces coelicolor

Sample Generation

Whole cell extracts (sonication)

 

Experimenter

Andrew Hesketh

Sample Processing

2D gel

 

Description

Changes in the proteome of strain M600 during growth and antibiotic production in liquid medium

Mass Spectrometry

MALDI-ToF

   

In Silico Analysis

Peptide mass fingerprint searches (MASCOT)

5

Organism

Mus Musculus BALB/C

Sample Generation

Exfoliated jejunal epithelium prepared as per Bjerknes & Cheng, Anat Rec 1981, 199, 565.

 

Experimenter

Alan Pemberton, Pamela Knight

Sample Processing

2D gel

 

Description

Trichinella spiralis infection in mice induces alterations in the proteome of the small mucosal epithelium

Mass Spectrometry

MALDI-ToF

   

In Silico Analysis

Peptide mass fingerprint searches (MASCOT, MS-FIT)

6

Organism

Homo sapiens

Sample Generation

Gel image analysis data from 12 patients, used to assign spot picks from a preparative 2D gel

 

Experimenter

Tony Whetton, Caroline Evans

Sample Processing

2D gel

 

Description

Proteomics can identify prognostic markers in CLL disease progression

Mass Spectrometry

MALDI-ToF

   

In Silico Analysis

Peptide mass fingerprint searches (MASCOT)

7

Organism

Saccharomyces cerevisiae

Sample Generation

Saccharomyces cerevisiae, strain YMK36, butanol – and butanol +

 

Experimenter

Kathleen Carroll

Sample Processing

2D gel

 

Description

Effect of butanol stress on yeast

Mass Spectrometry

MALDI-ToF

   

In Silico Analysis

Peptide mass fingerprint searches (MASCOT)

8

Organism

Saccharomyces cerevisiae

Sample Generation

 
 

Experimenter

Kathleen Carroll

Sample Processing

2D gel

 

Description

Mapping Heat shock proteins in Saccharomyces cerevisiae

Mass Spectrometry

MALDI-ToF

   

In Silico Analysis

Peptide mass fingerprint searches (MASCOT)

9

Organism

T. bruceii

Sample Generation

Gel pieces

 

Experimenter

Sarah Hart

Sample Processing

Bands from 1D gel

 

Description

Trypanosome Flagella

Mass Spectrometry

MALDI-ToF, LC MS/MS

   

In Silico Analysis

Mascot MS/MS