Four transmembrane domains within Acdp4 protein. Transmembrane domains were predicted by the TMHMM program http://www.cbs.dtu.dk/services/TMHMM-2.0/. The plot shows the posterior probabilities of inside /outside/TM helix. At the top of the plot (between 1 and 1.2) the N-best prediction is shown. The plot is obtained by calculating the total probability that a residue sits in helix, inside, or outside summed over all possible paths through the model.