Figure 1

Detection of brain specific splicing in gene LMO7. The top part of the figure is a visualization of gene LMO7 in SpliceNest, showing parts of three transcripts with exons displayed as red blocks, connected by lines representing introns. The middle exon of the top transcript (Hs5978.1) is missing in the second transcript (Hs5978.2) and is therefore highlighted as an alternative splice event (green bar). The boundaries corresponding to this exon as well as the corresponding intron are visualized as vertical lines in the GeneNest database (left and right box respectively). Both regions are covered by several ESTs depicted by horizontal arrows with corresponding tissues encoded in coloured rectangles towards the left of each EST. Upon comparing the tissue distribution of these alternative regions it is evident that the middle exon of transcript Hs5978.1 is covered by ESTs derived from several tissues, while the corresponding exon junction that lacks this middle exon, in transcript Hs5978.2, is represented by ESTs derived from brain only, thereby revealing this as a brain specific splice event.