Table 2 Information contained in a comprehensive cross list of soybean unigene clone IDs. Shown are various identifiers and annotations for 27,513 reracked cDNAs used in microarray construction. The full list is provided with arrays and available upon request.
Cross List Identifiers (for each cDNA clone) | Example (one of 27,543 cDNAs) | Comments |
|---|---|---|
Reracked Clone ID | Gm-r1021-12 | The individual cDNA clone ID in the 384-well destination plates after reracking or rearraying of the selected clones from the cDNA source library plates. |
Reracked Plate ID | Gm-r1021 #1 | The 384-well reracked plate name in increments of 384 (ie., 1, 385, 769, etc.) |
Reracked row_column position | A12 | Position of the clone in the 384-well reracked plate |
Reracked 3' Keck Sequence ID | GM210001A21A6 | Sequence identifier assigned by the Keck Center for the 3' EST |
Reracked 3' Genbank Accession | AW348131 | Genbank assigned accession number for the 3' EST |
Reracked 3' Annotation | glutathione S-transferase GST 22 [Glycine max] | Top BLASTX hit for the 3' EST, at E10-6 or lower |
Source Clone ID | Gm-c1004-464 | The individual cDNA clone ID in the 384-well source plate. |
Source Plate ID | Gm-c1004 #385 | The 384-well source plate name in increments of 384 (ie., 1, 385, 769, etc.) |
Source row_column position | D8 | Position of the clone in the 384-well source plate |
Source WashU Sequence ID | sa26h04.y1 | Sequence identifier assigned by Washington University, 5' EST |
Source 5' Genbank Accession | AI442436 | Genbank assigned accession number for the 5' EST |
Source 5' Annotation | glutathione S-transferase GST 22 [Glycine max] | Top BLASTX hit for the 5' EST at E10-6 or lower |
Source Library | Gm-c1004 | Name of the cDNA source library |
Cultivar/Genotype | Williams | Specific information on the soybean variety or genotype |
Tissue/Developmental Stage | Entire roots of 8-day old seedlings | Tissue/organ system/stage from which the cDNA library was constructed |