Lifespan reduction in Drosophila submitted to paraquat-, H
- and tunicamycin-induced stress 3-5 day-old Canton S wild type males were placed at t = 0 by groups of 30 in vials containing 15mM paraquat (P15: ●), 5 mM paraquat (P5: ◆), 1% H2O2 (H1: △) or 12 μM tunicamycin (T12: □). Dead flies were counted twice a day to determine survival. 3 vials of 30 individuals were used for each condition. When no toxic compound had been incorporated in the medium, more than 90% survival was observed at t = 120 h (not shown). Similar average lifespan was observed for P5, H1 and T12 around t = 80 h while it was significantly reduced in P15. Arrow indicates the time (t = 24 h) at which flies were collected for RNA extraction. Note the 20% lethality observed at this time for P15 condition. Dead flies were discarded before RNA extraction.