Table 1 Correlations between liver/brain RQ ratios of amplified vs. unamplified RNAs. For each correlation, n is the number of PCR results retained after filtering the amplification efficiency [22]. The correlation coefficient (r) is shown along with its 95% confidence interval (CI). Each correlation was significant at p < 0.0001. A cross-correlation matrix showing all relationships between samples is available at http://cord.rutgers.edu/amplification/index.html
From: Evaluation of sense-strand mRNA amplification by comparative quantitative PCR
n* | r ‡ | 95% CI | p | |
|---|---|---|---|---|
MessageAmp™ | 121 | 0.80 | 0.74 To 0.85 | <0.0001 |
Ovation™ | 112 | 0.82 | 0.76 To 0.86 | <0.0001 |
SenseAmp™ Version 1 | 118 | 0.87 | 0.83 To 0.90 | <0.0001 |
SenseAmp™ Version 2 | 121 | 0.88 | 0.85 To 0.91 | <0.0001 |
SenseAmp™ Version 1–2 | 121 | 0.90 | 0.87 To 0.93 | <0.0001 |
2 Rounds Version 1–2 | 121 | 0.89 | 0.85 To 0.92 | <0.0001 |
SenseAmp™ on degraded RNA | 121 | 0.94 | 0.92 To 0.96 | <0.0001 |