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Table 1 Correlations between liver/brain RQ ratios of amplified vs. unamplified RNAs. For each correlation, n is the number of PCR results retained after filtering the amplification efficiency [22]. The correlation coefficient (r) is shown along with its 95% confidence interval (CI). Each correlation was significant at p < 0.0001. A cross-correlation matrix showing all relationships between samples is available at http://cord.rutgers.edu/amplification/index.html

From: Evaluation of sense-strand mRNA amplification by comparative quantitative PCR

  n* r 95% CI p
MessageAmp™ 121 0.80 0.74 To 0.85 <0.0001
Ovation™ 112 0.82 0.76 To 0.86 <0.0001
SenseAmp™ Version 1 118 0.87 0.83 To 0.90 <0.0001
SenseAmp™ Version 2 121 0.88 0.85 To 0.91 <0.0001
SenseAmp™ Version 1–2 121 0.90 0.87 To 0.93 <0.0001
2 Rounds Version 1–2 121 0.89 0.85 To 0.92 <0.0001
SenseAmp™ on degraded RNA 121 0.94 0.92 To 0.96 <0.0001
  1. * number of valid samples shared with unamplified control
  2. Pearson correlation coefficient