Figure 6

Methylation state of ERTBV in the genomic DNAs from the Oryza AA-genome species. Each genomic DNA was digested with Msp I (M) or Hpa II (H) as described in Materials and Methods. Differential hybridization patterns between the Msp I and Hpa II digests observed in O. sativa and O. rufipogon show heavier methylation compared to the others, and O. longistaminata indicates possession of methylated ERTBV segments in the genome.