Figure 1

Array fabrication and gene annotation (A) False-colour image generated from a bovine-Cy5, human-Cy3 hybridisation. False colour images were generated using the programme ScanAlyze, version 2.44 http://www.rana.lbl.gov/EisenSoftware.htm. The full array (24 × 25 spotting pattern) consists of 16 blocks with each gene spotted 5 times per block therefore 80 potential data points present for expression analysis per gene. A blow-up of 4 blocks illustrating the Chip design is presented. The ACTIN cDNAs acting as guide-dots can be seen as intense yellow spots demarcating each block. In addition, the majority of spots appear yellow due to similar expression levels of the orthologous genes. (B) Functional annotation of the 349 genes as set out by the Gene Ontology Consortium http://rana.lbl.gov/EisenSoftware.htm.The proportion of these genes within each functional / biological annotation is represented on the Y-axis and the annotation on the X-axis. (C) Chromosomal distribution of the 349 genes. The number of genes within each chromosome is represented on the Y-axis and the chromosome numbers on the X-axis.