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Figure 5 | BMC Genomics

Figure 5

From: Cross-species hybridisation of human and bovine orthologous genes on high density cDNA microarrays

Figure 5

Independent verification of array results by semi-quantitative RT-PCR (A) Ethidium bromide stained gel illustrating independent verification of differential gene expression by semi-quantitative RT-PCR. PCR reactions were carried out in 50 μl volumes, loaded in the order H- (human), B- (bovine) and VE- (water as negative control) then resolved on a 2 % agarose gel as described in Materials and Methods. Each panel consist of genes of the same functional category – listed on the left hand side. A 100 bp ladder (Invitrogen) was used to confirm the sizes (Table 1) of the PCR products. (B) A graphical illustration of the comparison of the expression levels of the orthologous genes as deduced by the microarray analysis and semiquantitative RT-PCR.

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