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Table 1 Summary of primer sequences, annealing temperatures and size of amplicons. Derivation of primer sequences: H and B denote human and bovine respectively. The nucleotide positions of the primer sequences are in parenthesis. Nucleotides highlighted in bold denote differences in the orthologous gene sequences.

From: Cross-species hybridisation of human and bovine orthologous genes on high density cDNA microarrays

Genes

Primer sequences (5' – 3')

Annealing temperature (°C)

Fragment size (bp)

Accession numbers

CDC27

(1226–1250) H

TATTACATCTCCCCCAAACGCACTG

54

311

NM_001256 H

CB170694 B

 

(1512–1536) H

CCATTTCACGAAGAAGGCTCATCAA

   

CDC6

(89–113) B

TCCCAAGCGGGTTGGTG TTATTCAC

54

208

NM_001254 H

BF600055 B

 

(272–296) B

GCGACAGACTG TACTGTAGGCTTCA

   

PCNA

(195–217) B

GAGGCGCT TAAGGATCTCAT CAA

54

382

AF527838 H

CB531519 B

 

(554–576) B

ATTCACCAGAAGGCATCTTTACT

   

SLC11A3

(38–61) B

ACCCCTGGAGGGAACTCATCTAAT

57

276

AF215636.1 H

BG689712 B

 

(290–313) B

GCTGATGCTCCCATCAAAATACTG

   

VDAC2

(652–675) B

CCTC GGTTGTGATGTTGACTTTGA

57

448

NM_003375.1 H

TC152811 B

 

(1076–1099) B

GTGGCCTCCAGCATTAATGCTCTT

   

CALNEXIN

(1054–1078): H

GCTGGTTAGATGATGAGCCTGAGTA

56

237

NM_001746.1 H

BM431118 B

 

(1266–1290): H

TCCTG GGTTTCCAGATTCCCTGGTA

   

β-ACTIN

441–461) H

GTTGCTATCCAGGCTGTGCT

60

469

NM_001101 H

NM_173979 B

 

(890–910) H

CGGATGTCCACGTCACACTT

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