Output from SFINX for (A) C. elegans protein C36B1.12 and (B) one of its assigned human orthologs Q8TCT8. The overall membrane topology of the two proteins is very similar. The consensus from the different topology predictors is nine transmembrane (TM) regions, a N-terminal signal peptide, and a >150 amino acids non-cytoplasmic N-terminal region. Conserved aspartic acid residues are marked with an asterisk (residues 433 and 516 for C36B1.12; residues 351 and 412 for Q8TCT8). Phobius is the only program used that predicts both TM regions and N-terminal signal peptides. The other programs are designed to only detect TM regions, and therefore, they commonly mistake the signal peptide for a TM region. Numbers on the horizontal axis indicate amino acid positions. Color coding: black = TM region, white = non-cytoplasmic, gray = cytoplasmic, striped = N-terminal signal peptide predicted by Phobius.