Table 2 RNAi phenotype and major tissues of gene expression for C. elegans orthologs. Abbreviations: RNAi phenotype: clear (Clr), dumpy (Dpy), larval arrest (Lva), ruptured (Rup), sterile (Ste), wildtype (WT). Gene expression: body wall muscle (bwm), commissures (c), excretory system (exc), gonad (g), hypodermis (h), hypodermal seam cells (hs), intestine (i), neuronal (n), pharyngeal muscle (phm), rectal epithelial cells (re), spermatheca (s), vulva (v), ventral nerve cord (vnc). A limitation when extrachromosomal array transgenes are used is that expression in the germ line is not possible to evaluate. No transgenic lines could be generated for T11F9.2, H13N06.5 and T04A8.12. Possible reasons for this could be that the injected DNA concentration was too low or that the sequence was toxic. In either case, the extrachromosomal array formed may not have been sufficiently large to be inheritable [46]. The transgenic lines for ZK858.6 and F14F3.3 showed no expression of gfp. This might be caused by conditional gene expression, germline silencing or absence of the promoter::gfp fusion from the inheritable extrachromosomal array [46].
From: Functional characterization in Caenorhabditis elegans of transmembrane worm-human orthologs
C. elegans orthologs | RNAi phenotype | Major tissues of gene expression |
|---|---|---|
C30H6.2 | WT | h, phm |
T11F9.2a, T11F9.2b | WT | No transgenic line |
H13N06.5 | WT | No transgenic line |
T28F3.3 | WT | h, i, n, v, vnc |
T01D3.5 | WT | hs |
F40F9.1a, F40F9.1b | WT | bwm, c, h, n, phm, vnc |
F40F9.2 | WT | exc, n, phm |
F08F1.7 | WT | h, n, phm, re, s, v |
ZK858.6a, ZK858.6b | WT | No expression |
F14F3.3 | WT | No expression |
R155.1a, R155.1b | Dpy | bwm, h, i, phm |
C36B1.12 | WT | i, n |
sft-4 | Clr, Lva, Rup, Ste | bwm, h, i, n, phm, v |
D2013.10 | WT | bwm, h, i, n, s, v |
T04A8.12 | WT | No transgenic line |
Y6B3B.10 | WT | phm |
ZK721.1 | WT | bwm, g, h, i, n, phm, s, v |