Figure 5
From: Genomic structure and cloning of two transcript isoforms of human Sp8
Expression of Sp8 mRNA in human and mouse adult tissues determined by RT-PCR using primers located in the 3'UTR. A. Ethidium bromide stained agarose gel of PCR products from human tissues. The lanes are: skeletal muscle, muscle; small intestine, intestine; peripheral blood leukocytes, leukocyte; negative control, -c and 100 bp ladder, m. The expression of beta-actin mRNA, a housekeeping gene, in the same samples is shown below. B. RT real time PCR of murine Sp8 mRNA expression normalised to that of beta-actin.