Figure 6
From: Genomic structure and cloning of two transcript isoforms of human Sp8
Expression of Sp8 mRNAs in human osteoblast-like cells by reverse transcriptase PCR. Amplicons were run on an ethidium bromide stained agarose gel. The amplicons were: Sp8 long protein isoform, Sp8L; Sp8 short protein isoform, Sp8S; Runx2/Cbfa1, Runx2 and the housekeeping gene beta-actin. The sizes of the amplicons are indicated. The lanes are: primary adult osteoblasts, AO; HOS osteosarcoma cell line, HS; MG63 osteosarcoma cell line, MG; primary craniofacial osteoblasts, CF; primary foetal osteoblasts, FO; primary adult chondrocytes, CH; negative control, (-) and 100 bp markers, m.