Representation of small and large transcripts in aRNA generated by optimised purification protocol. (A) 2.5% agarose gel of R38b RT-PCR product using HCC-1187 aRNA as template. (B) 0.7% agarose gel of p532 RT-PCR product using HCC-1187 aRNA as template. (C) Box-plot for A values (average log2 intensity) of three transcript size categories (Long: > 7,000 bases, Medium: 500–7,000 bases, Short: <500 bases). (D) Coefficients of repeatability (CR) as described by Jenssen et al , are demonstrated in 8 replicate data sets for three transcript size categories.