Figure 8

Comparison of the human TFIIH P62 subunit with the hypothetical MAL3P7.42 from P. falciparum. Significant similarities were detected for the region including two BSD domains (boxed, panel B). These were supported at the 2D level by comparison of the HCA plots (see Fig. 2 for explanation), where cluster similarities are shaded in grey and identities are shown in white on a black background. Marginal similarities observed in PSI-BLAST are located within the C-terminal parts of the proteins (ranging from ~250 to the C-terminus (human) and from ~aa 370 to the C-terminus (P. falciparum)). These were also supported at the 2D level, as illustrated here on a segment in the most distal C-terminal part of the two proteins (panel C). Upstream of the BSD domain, cluster similarities together with sequence identities can be observed in the most proximal N-terminal part of the protein sequences, corresponding to a pH domain in human p62 (panel A). Secondary structures, as observed in the experimental structure of human p62 (pdb 1pfj; [80]), are reported up to its sequence. The corresponding 1D alignment is shown in panel D.