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Table 1 Cross-fluorescence of tested dyes. Fluorescence of dyes at scanner settings of all dyes expressed as a percentage of fluorescence at its own settings (the latter was set to 100%).

From: Application of four dyes in gene expression analyses by microarrays

dye Alexa 488 Alexa 594 Cy3 Cy5
settings     
Alexa 488 100.0 0.0 1.1 0.0
Alexa 594 0.9 100.0 1.3 13.0
Cy3 0.0 2.2 100.0 0.0
Cy5 0.1 0.1 0.2 100.0