Table 1 Cross-fluorescence of tested dyes. Fluorescence of dyes at scanner settings of all dyes expressed as a percentage of fluorescence at its own settings (the latter was set to 100%).
From: Application of four dyes in gene expression analyses by microarrays
dye | Alexa 488 | Alexa 594 | Cy3 | Cy5 |
|---|---|---|---|---|
settings | Â | Â | Â | Â |
Alexa 488 | 100.0 | 0.0 | 1.1 | 0.0 |
Alexa 594 | 0.9 | 100.0 | 1.3 | 13.0 |
Cy3 | 0.0 | 2.2 | 100.0 | 0.0 |
Cy5 | 0.1 | 0.1 | 0.2 | 100.0 |