Application of four dyes in gene expression analyses by microarrays

BMC Genomics

Table 5b Performance of a dye combination in revealing modulated genes in B[a]P (10 μM) treated HepG2 cells using a cDNA array.

	Cy3-Cy5	A594-Cy3	Cy5-A488	A488-A594
Cy3-Cy5	29*
A594-Cy3	23	58
Cy5-A488	6	10	16
A488-A594	26	39	13	74

* In bold the intersection of two gene lists indicating the modulated genes for a dye combination as percentage of all modulated genes (31 genes) found by analysis of all dye combinations combined. In italics the intersection of three gene lists indicating the modulated genes for two dye combinations as percentage of all modulated genes found for all dye combinations combined. The first dye was used for B[a]P treatment, the second for the control.

ISSN: 1471-2164