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Figure 2 | BMC Genomics

Figure 2

From: A systematic search for new mammalian noncoding RNAs indicates little conserved intergenic transcription

Figure 2

Screening, selection, and confirmation of novel ncRNA predictions. (a) Total RNA from 16 tissues was hybridized to custom Agilent microarrays containing probes to the QRNA predictions. (b) Most intensity measurements overlapped negative-control intensities (both random probe sequences and probes corresponding to randomly sampled intergenic regions), although a right-tailed distribution overlapping rRNA levels indicated detection of potential abundant novel transcripts. (c) Expanded y-axis region from (b), axes denote absolute probe intensity counts. (d) Sample schematic of microarray spots corresponding to a transcript that was tested further by northern analysis. (e) 55 transcripts in total were screened by Northern analysis.

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