Figure 2From: A systematic search for new mammalian noncoding RNAs indicates little conserved intergenic transcriptionScreening, selection, and confirmation of novel ncRNA predictions. (a) Total RNA from 16 tissues was hybridized to custom Agilent microarrays containing probes to the QRNA predictions. (b) Most intensity measurements overlapped negative-control intensities (both random probe sequences and probes corresponding to randomly sampled intergenic regions), although a right-tailed distribution overlapping rRNA levels indicated detection of potential abundant novel transcripts. (c) Expanded y-axis region from (b), axes denote absolute probe intensity counts. (d) Sample schematic of microarray spots corresponding to a transcript that was tested further by northern analysis. (e) 55 transcripts in total were screened by Northern analysis.Back to article page