Phylogenetic tree of the FBG domains of the FREP family in A. gambiae . Phylogenetic relationships of the FBG domains are shown. The seed alignment used for constructing the tree was the multiple alignment sequences shown in Fig. 1. Maximum-likelihood approach was used to construct the tree with the proml program of the PHYLIP package, which uses the Jones-Taylor-Thornton model of change between amino acids and a Hidden Markov Model (HMM) method of inferring different rates of evolution at different amino acid positions. The FBG domains of each FREP are denoted by their gene name in GenBank.