Figure 4

Comparison of the mRNA levels of the cys genes of the C. glutamicum wild-type and a cys I transposon mutant. Total RNA was isolated from cells grown in MMS with 1 mM L-cysteine as sulphur source and the relative transcription levels were determined using real-time RT-PCR to quantify the mRNAs of the displayed genes. Small black bars inside the arrows representing the genes indicate the position of the internal fragments amplified in the real-time RT-PCR.