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Table 4 Bacterial strains and plasmids

From: Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction

Name Relevant genotype/information a Source/reference
E. coli   
DH5αMCR F- endA1 supE44 mcrA thi-1 hsdR17 λ- recA1 relA1 Δ (lacZYA-argF )U169 (Φ 80dlacZΔM15 ) gyrA96 deoR Δ (mrr-hsdRMS-mcrBC ) [40]
AB462, CGSC 462 F- DE (gpt-proA )64 lacYl galK2 (Oc)hisG4 (Oc) λ- cysC9 xylA5 mtl-1 thi-1 CGSCb
JM226, CGSC 5468 F- pro-0 lac -tsx -? galT47 trp-74 his -cysH57 xyl argA9999 rpsL -(strR) mal -ilvA -mtl - CGSC, [47]
JM221, CGSC 5745 F- pro-50 lac- tsx -galT47 trp-74 his-97 cysD91 argA-rpsL--(strR) mat xyl-ilvA-mtl- CGSC, [47]
JM246, CGSC 5747 F- λ- cysI53(Am)IN(rrnD-rrnE)1 rph-1 CGSC, [47]
C. glutamicum   
ATCC 13032 Wild type, Nxr ATCCc
CR018 Δcg3112 (cysZ) this study
CR019 Δcg3113 (cysY) this study
CR020 Δcg3114 (cysN) this study
CR021 Δcg3115 (cysD) this study
CR022 Δcg3116 (cysH) this study
CR023 Δcg3117 (cysX) this study
CR024 Δcg3118 (cysI) this study
CR025 Δcg3119 (fpr2) this study
CR026 cg3118::IS6100, pAT6100 this study
Plasmids   
pK18mobsacB sacB, lacZa, Kmr, mcs mobilizable vector, allows for selection of double-crossover in C. glutamicum [35]
  mobilizable vector, allows for selection of double-crossover in C. glutamicum  
pEC-XK99E Ptrc, lacIq, Kmr inducible E. coliC. glutamicum – shuttle expression vector [48]
pAT6100 IS61 00, Kmr [36]
pCR018d pK18mobsacB carrying cg3112 deld this study
pCR018e pEC-XK99E carrying cysZ eve this study
pCR019d pK18mobsacB carrying cg3113 del this study
pCR019e pEC-XK99E carrying cysY ev this study
pCR020d pK18mobsacB carrying cg3114 del this study
pCR020e pEC-XK99E carrying cysDN ev this study
pCR021d pK18mobsacB carrying cg3115 del this study
pCR022d pK18mobsacB carrying cg3116 del this study
pCR022e pEC-XK99E carrying cysH ev this study
pCR023d pK18mobsacB carrying cg3117 del this study
pCR023e pEC-XK99E carrying cysX ev this study
pCR024d pK18mobsacB carrying cg3118 del this study
pCR024e pEC-XK99E carrying cysI ev this study
pCR025d pK18mobsacB carrying cg3119 del this study
  1. ar superscript indicates resistance. nx, Nalidixic acid; Km, Kanamycin
  2. bCGSC; E. coli Genetic Stock Center, Yale University, New Haven, CT
  3. cATCC; American Type Culture Collection, Rockville, MD
  4. dthe postfix del indicates inserts used for targeted gene deletion
  5. ethe postfix ev indicates genes preceded by an artificial RBS