Phylogram of NEK kinases. NEK sequences from human (Hs), S. cerevisiae (Sc), C. elegans (Ce), D. melanogaster (Dm), P. falciparum (Pf) and T. brucei (Tb) were analyzed using MRBAYES. TbCK2A1 was used as an outgroup. Nodes with a bootstrap value greater than 0.95 are marked by a dot, values ranging from 0.7–0.95 are indicated numerically. Similar results were obtained with PAUP*. Two NEK kinases had recognizable PH domains that did not achieve the Pfam HMM search cutoff (Tb04.24M18.60, and Tb08.10K10.710).