Figure 1

A) Comparison of most expressed tags among different SAGE libraries. Normalized expression levels (TPM) are similar between libraries with different total sequenced tags, indicating comparable messenger abundance among top expressed genes. Expression levels were obtained from SAGEmap website http://wwwncbinlmnihgov/projects/SAGE/indexcgi?cmd=expsetup. ^aTPM: Tags per million. Normalization to compare libraries with different numbers of sequenced tags. TPM is obtained by the following formula [(Tag frequency)(1000,000)/Total No. of sequenced tags]. ^bDN: digital northern, indicating gene expression level for a specific gene in a library. B. Graphical representation of expression levels (TPM) for three constitutive genes in several normal (N) and tumoral (T) tissues. Brain tissue libraries: SAGE BB542 whitematter (N) and SAGE Brain medulloblastoma B 98 04 P117 (T). Breast: SAGE Breast normal organoid (N) and B SAGE Breast carcinoma epithelium AP DCIS6 (T). Gastric: SAGE normal gastric body epithelial (N) and SAGE Hiroshima GC W246T (T). Liver: SAGE normal liver (N) and SAGE Liver cholangiocarcinoma B K2D (T). Kidney: SAGE Duke Kidney (N) and SAGE_Kidney_carcinoma_B_D2 (T). Colon: SAGE NC2 (N) and SAGE Tu98 (T). Prostate: SAGE PR317 normal prostate (N) SAGE PR317 prostate tumor (T). Lung: SAGE normal lung (N) and SAGE Lung adenocarcinoma MD L10 (T). Expression levels are indicated as tags per million.