Characterization of the global profile of genes expressed in cervical epithelium by Serial Analysis of Gene Expression (SAGE)

Table 4 Oligonucleotides sequences used in this work.

Gene	Sense (5'→3')	Antisense (5'→3')	Annealing temperature (°C)^a	Product size (bp)	Reference
HPV*	GCMCAGGGWCATAAYAATGG	CGTCCMARRGGAWACTGATC	55	450	[36]
S100 A8	ATGCCGTCTACAGGGATGAC	ACGCCCATCTTTATCACCAG	58	160	This paper
S100 A9	TCAGCTGGAACGCAACATAGA	TCAGCTGCTTGTCTGCATT	56	205	This Paper
SPRR3	TTCCACAACCTGGAAACACA	TTCAGGGACCTTGGTGTAGC	55	174	This paper
NICE-3	ACGGCTATGAAACAGCCCGCTA	GCACATTGCAACTGACTGGCTT	57	330	This paper
NICE-4	ACGGAATCCAATGAGGAAGGCA	TCAGTATTGGCTGGCTCTGCAT	57	294	This paper
GAPDH	CATCTCTGCCCCCTCTGCTGA	GGATGACCTTGCCCACAGCCT	60	205	[38]

^aTm was calculated using primerquest program from [39]; however, it was necessary to adjust Tm in some cases.

ISSN: 1471-2164