Figure 2From: Construction and validation of a Bovine Innate Immune MicroarrayEffect on the signal reported from DNA elements of varying quantity, length and position within the target transcript. The mean background corrected signal intensity was measured for each β-actin and GAPDH control element on a microarray hybridised with labelled cDNA from ConA stimulated PBLs. (a) Signal reported by β-actin (◆) and GAPDH (■) elements as a function of the quantity of spotted DNA. The error bars denote one standard deviation of the mean. (b) Signal reported by β-actin (◆) and GAPDH (■) elements with DNA lengths ranging from 200 to 1500 bp. (c) Signals reported by β-actin (black) and GAPDH (unshaded) elements of constant DNA length but positioned at the 3'-end, mid-region or 5'-end of the respective target transcripts.Back to article page