Slide to slide reproducibility of the Bovine Innate Immune Microarray. (a) Schematic diagram of the experimental design. Each arrow represents one microarray slide with the arrow direction indicating the cDNA labelling from Cy5 to Cy3-labelled cDNA. Bovine PBLs were cultured for 24 h with or without ConA (5 μg/ml). (b) An example of slide-to-slide reproducibility depicted as a scatter plot of the signal ratio on slide 1 vs slide 3. The signal ratio was calculated by dividing the background corrected signal for ConA stimulated PBLs by the background corrected signal for unstimulated PBLs.