Figure 4

Estimation of the limit of signal detection for the Bovine Innate Immune Microarray. The Lucidea Universal RNA mix was used in both Cy3 and Cy5 labelling reactions with specific transcripts present at a concentration range of 0.1 to 3000 pg per μg of total sample RNA. The mean background corrected signal, reported from each corresponding Lucidea Calibration control element printed on the microarray is plotted as log₂ (signal) in Cy3 (■) and Cy5 (◆) dye channels. Error bars represent one standard deviation from the mean.