Figure 5

Time course of ConA activation of bovine peripheral blood lymphocytes. (a) Schematic diagram of the experimental design. Each arrow represents one microarray slide with the arrow direction indicating the cDNA labelling from Cy5 to Cy3-label. Bovine PBLs were stimulated with ConA (5 μg/ml) for 0.5, 2, 4, 8 or 24 h. cDNA from the treated cells were compared to cDNA from unstimulated cells. (b) MA plots of microarray data from the ConA activation time course (dye swap replicates are not shown). Labelled cDNA from bovine PBLs treated with ConA (Cy5) were compared to labelled cDNA from unstimulated cells (Cy3). The X-axis shows the total signal intensity for each element present on the microarray (calculated as 1/2*((log₂(Cy5) + log₂(Cy3))). Y-axis shows the log₂ (signal ratio) (log₂(Cy5/Cy3)). Elements with a log₂ (signal ratio) greater than zero represent transcripts which are more abundant in the ConA activated PBL sample.