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Figure 7 | BMC Genomics

Figure 7

From: Construction and validation of a Bovine Innate Immune Microarray

Figure 7

Validation of gene expression profiles by quantitative real time RT-PCR (qRT-PCR). The three gene expression profiles discovered by microarray analysis were validated by qRT-PCR with genes representative of each cluster. The log2 (fold change) was calculated for both microarray data () and qRT-PCR data (■) at each treatment time point relative to that in unstimulated cells. TNF represents the gene expression profile of Induction 1; TNFRSF9, IL2RA and CCL3L1 represent the Induction 2 profile and THBS represents the Suppression 1 profile.

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