Figure 7From: Construction and validation of a Bovine Innate Immune MicroarrayValidation of gene expression profiles by quantitative real time RT-PCR (qRT-PCR). The three gene expression profiles discovered by microarray analysis were validated by qRT-PCR with genes representative of each cluster. The log2 (fold change) was calculated for both microarray data (â—†) and qRT-PCR data (â– ) at each treatment time point relative to that in unstimulated cells. TNF represents the gene expression profile of Induction 1; TNFRSF9, IL2RA and CCL3L1 represent the Induction 2 profile and THBS represents the Suppression 1 profile.Back to article page