Figure 8

Cross-species hybridisation of an ovine sample to the Bovine Innate Immune Microarray. (a) Schematic diagram of the experiment which used unstimulated ovine and bovine PBLs cultured for 24 h. Each arrow represents one microarray slide where the arrow direction indicates the cDNA labelling from Cy5 to Cy3-label. (b) Scatter plots of microarray data as log₂ (signal). The upper and middle panels show the variation observed when comparing signals from an identical cDNA source which was labelled with both Cy3 and Cy5 dye; bovine/bovine (upper panel) and ovine/ovine (middle panel). The lower panel shows a scatter plot of the log₂ (signal) from ovine PBL cDNA compared to the log₂ (signal) from bovine PBL cDNA.