Table 3 Oligonucleotide primer sequences for qRT-PCR validation of microarray results
From: Construction and validation of a Bovine Innate Immune Microarray
Gene name (Symbol) | Accession number | Forward | Reverse | Amplicon size (bp) |
|---|---|---|---|---|
Acidic Ribosomal Protein Large P0 (RPLP0) | NM_001012682 | CAACCCTGAAGTGCTTGACAT | AGGCAGATGGATCAGCCA | 220 |
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) | AF077815 | CCTGGAGAAACCTGCCAAGT | GCCAAATTCATTGTCGTACCA | 226 |
Tumour Necrosis Factor alpha (TNF) | NM_173966 | CTGGTTCAGACACTCAGGTCCT | GAGGTAAAGCCCGTCAGCA | 183 |
Interferon gamma (IFNG) | NM_174086 | GTGGGCCTCTCTTCTCAGAA | GATCATCCACCGGAATTTGA | 234 |
Chemokine (C-C motif) ligand 3-like 1 (CCL3L1) | NM_174511 | GGTCTTCTCGGCACCATTT | CCAGGTCGGTGATGTATTCC | 209 |
Interleukin 2 receptor alpha (IL2RA) | NM_174358 | ACCATGATAAACTGCGACTGC | GGTTGGTAAGAAAGTTCCACTCC | 513 |
Tumor Necrosis Factor Receptor Super Family Member 9 (TNFRSF9) | NM_001561 | AAATCCTGCAGTGATCGTGTCC | CTTCTTCAGCAGCCCTGGAAT | 189 |
Thrombospondin (THBS) | NM_174196.1 | CCAATCCTGACCAGAAGGAC | TGGCGTACAACCCAGTTAGG | 202 |